Anti-ARRDC3 antibody (ab64817)
Key features and details
- Rabbit polyclonal to ARRDC3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ARRDC3 antibody
See all ARRDC3 primary antibodies -
Description
Rabbit polyclonal to ARRDC3 -
Host species
Rabbit -
Specificity
ab64817 detects endogenous levels of total ARRDC3 protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A synthetic peptide derived from the C-terminal of human ARRDC3.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab64817 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARRDC3 antibody (ab64817)All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO205 cells
Lane 3 : Extracts from COLO205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
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Western blot - Anti-ARRDC3 antibody (ab64817)All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution
Lane 1 : Rat brain lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse brain lysates with immunizing peptide at 5 µg
Predicted band size: 46 kDa
Observed band size: 46 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARRDC3 antibody (ab64817)IHC image of ab64817 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64817, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-ARRDC3 antibody (ab64817)ICC/IF image of ab64817 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64817, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
