Anti-ARPC2 antibody (ab11798)
Key features and details
- Goat polyclonal to ARPC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARPC2 antibody
See all ARPC2 primary antibodies -
Description
Goat polyclonal to ARPC2 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Zebrafish
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Immunogen
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
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Positive control
- WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARPC2 antibody (ab11798)Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6). -
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
