Anti-ARHGAP29 antibody (ab85853)
Key features and details
- Rabbit polyclonal to ARHGAP29
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP29 antibody
See all ARHGAP29 primary antibodies -
Description
Rabbit polyclonal to ARHGAP29 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ARHGAP29 aa 1211-1261.
Database link: NP_004806.3 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
This product was previously labelled as Rho GTPase activating protein 29
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARHGAP29 antibody (ab85853)All lanes : Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ARHGAP29 antibody (ab85853)Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 µg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.