Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18138] to APOBEC3B - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free
    See all APOBEC3B primary antibodies

  • Description

    Rabbit monoclonal [EPR18138] to APOBEC3B - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250804 is the carrier-free version of ab184990. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250804 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

    Lane 1 : Human APOBEC3B fragment recombinant protein
    Lane 2 : Human APOBEC3G fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 1 minute


    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution

    Lane 1 : HepaRG-APOBEC3B tetracycline-inducible cell line (+/-)
    Lane 2 : HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-)
    Lane 3 : HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

    Predicted band size: 46 kDa
    Observed band size: 35,70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab184990, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Panel A: 20 s; Panel B: 1 min.

    Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.

    The 35 kDa band represents a known splice variant of APOBEC3B.

  • Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)
    Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (ab250804)

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