Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22929-208] to Annexin-4/ANXA4 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free
    See all Annexin-4/ANXA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22929-208] to Annexin-4/ANXA4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney, liver and lung lysate. HepG2, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 lysates. Mouse liver, kidney and lung lysate. Rat kidney lysate. IHC-P: Human pancreas, kidney, endometrium, mouse and rat stomach tissue. ICC/IF: HepG2 and NIH/3T3 cells. Flow Cyt: HepG2 and NIH/3T3 cells.

  • General notes

    ab259999 is the carrier-free version of ab256456 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab259999 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat stomach. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse stomach. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human endometrium (PMID: 16954445) is observed. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human kidney (PMID: 9720986) is observed. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in centroacinar cells and islets of human pancreas. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Flow Cytometry - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Flow Cytometry - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Annexin-4/ANXA4 with ab256456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Flow Cytometry - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Flow Cytometry - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Annexin-4/ANXA4 with ab256456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse pancreas (PMID: 25176043) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse pancreas (PMID: 25176043) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on rat kidney (PMID: 15145526) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse kidney (PMID: 15145526) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Annexin-4/ANXA4 with ab256456 at 1/100 5.2 µg/ml dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing nuclear, cytoplasmic and staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Annexin-4/ANXA4 with ab256456 at 1/100 5.2 µg/ml dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing nuclear, cytoplasmic and staining in HepG2 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256456).

  • Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)
    Anti-Annexin-4/ANXA4 antibody [EPR22929-208] - BSA and Azide free (ab259999)

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