Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)
Key features and details
- Rabbit polyclonal to Ankyrin repeat domain-containing protein 65
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ankyrin repeat domain-containing protein 65 antibody -
Description
Rabbit polyclonal to Ankyrin repeat domain-containing protein 65 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Ankyrin repeat domain-containing protein 65 aa 1-100.
Database link: E5RJM6 -
Positive control
- WB: RT-4 and U-251 MG whole cell lysates; ICC: RT4 whole cell lysates; IHC-P: Human Fallopian tube tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)
Immunohistochemical analysis of human Fallopian tube tissue labeling Ankyrin repeat domain-containing protein 65 (high expression) with ab204604 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)Immunocytochemical analysis of RT4 (Human urinary bladder cancer cell line) whole cells labeling Ankyrin repeat domain-containing protein 65 in the nucleoplasm with ab204604 at 2 µg/ml.
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Western blot - Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)All lanes : Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 41 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)Immunohistochemical analysis of human kidney tissue labeling Ankyrin repeat domain-containing protein 65 (low expression) with ab204604 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
