Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)
![Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)](https://www.abcam.com/ps/products/212/ab212053/Images/ab212053-331792-anti-ankyrin-erythroid-ank-antibody-s388a-10-western-blot.png "Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)")
Key features and details
- Mouse monoclonal [S388A-10] to Ankyrin erythroid/ANK
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Ankyrin erythroid/ANK antibody [S388A-10] -
Description
Mouse monoclonal [S388A-10] to Ankyrin erythroid/ANK -
Host species
Mouse -
Specificity
Does not cross-react with Ankyrin-B or Ankyrin-G.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Fusion protein corresponding to Human Ankyrin erythroid/ANK aa 1-1881.
Database link: P16157 -
Positive control
- WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S388A-10 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 206 kDaPrimary incubation 16 hours at 4°C.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)](https://www.abcam.com/ps/products/212/ab212053/Images/ab212053-331802-anti-ankyrin-erythroid-ank-antibody-s388a-10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053)4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Ankyrin erythroid/ANK (green, C) using ab212053 at 1/100 dilution in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
