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Neuroscience Cell Adhesion Proteins Membrane Proteins

Anti-AMIGO1 antibody [S86-36] (ab167439)

Anti-AMIGO1 antibody [S86-36] (ab167439)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [S86-36] to AMIGO1
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-AMIGO1 antibody [S86-36]
    See all AMIGO1 primary antibodies
  • Description

    Mouse monoclonal [S86-36] to AMIGO1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Fusion protein corresponding to Human AMIGO1 aa 554-574. (cytoplasmic C-terminus)
    Database link: NP_065754.2

  • Positive control

    • WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    S86-36
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Images

  • Western blot - Anti-AMIGO1 antibody [S86-36] (ab167439)
    Western blot - Anti-AMIGO1 antibody [S86-36] (ab167439)
    Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT

    Developed using the ECL technique.

    Predicted band size: 55 kDa


    Exposure time: 6 minutes


    Block: 2% BSA and 2% Skim Milk in 1X TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-AMIGO1 antibody [S86-36] (ab167439)
    Immunocytochemistry/ Immunofluorescence - Anti-AMIGO1 antibody [S86-36] (ab167439)

    4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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