Anti-AMIGO1 antibody [S86-36] (ab167439)
Key features and details
- Mouse monoclonal [S86-36] to AMIGO1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-AMIGO1 antibody [S86-36]
See all AMIGO1 primary antibodies -
Description
Mouse monoclonal [S86-36] to AMIGO1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Fusion protein corresponding to Human AMIGO1 aa 554-574. (cytoplasmic C-terminus)
Database link: NP_065754.2 -
Positive control
- WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S86-36 -
Isotype
IgG1 -
Research areas
Images
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Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg
Secondary
Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skim Milk in 1X TBST.
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4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.