Anti-alpha smooth muscle Actin antibody (ab265588)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to alpha smooth muscle Actin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-alpha smooth muscle Actin antibody
See all alpha smooth muscle Actin primary antibodies -
Description
Rabbit monoclonal to alpha smooth muscle Actin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human alpha smooth muscle Actin aa 1-50. The exact sequence is proprietary. NP_001135417.1
Database link: P62736 -
Positive control
- IHC-P: Human breast tissue. Human colon carcinoma tissue. ICC: IMR-90 cells. WB: IMR-90 and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
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Western blot - Anti-alpha smooth muscle Actin antibody (ab265588)All lanes : Anti-alpha smooth muscle Actin antibody (ab265588) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : A549 whole cell lysate at 50 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : IMR-90 whole cell lysate at 10 µg
Lane 5 : HepG2 whole cell lysate at 50 µg
Lane 6 : LNCaP whole cell lysate at 50 µg
Lane 7 : RKO whole cell lysate at 50 µg
Lane 8 : K562 whole cell lysate at 50 µg
Lane 9 : NIH/3T3 whole cell lysate at 50 µg
Lane 10 : TCMK whole cell lysate at 50 µg
Predicted band size: 42 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
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Immunocytochemistry - Anti-alpha smooth muscle Actin antibody (ab265588)Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
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Anti-alpha smooth muscle Actin antibody (ab265588)
