Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
![Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)](https://www.abcam.com/ps/products/190/ab190467/Images/ab190467-225603-anti-alpha-hemolysin-antibody-8b7-n-terminal-neutralising.jpg "Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)")
Key features and details
- Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal
- Suitable for: WB, Neutralising
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Overview
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Product name
Anti-alpha-hemolysin antibody [8B7] - N-terminal -
Description
Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal -
Host species
Mouse -
Specificity
ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region. -
Tested applications
Suitable for: WB, Neutralisingmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Synthetic peptide corresponding to alpha-hemolysin (N terminal). Polypeptide sequence of Staphylococcus aureus alpha-hemolysin targeting the N terminus of the mature toxin.
Database link: P09616 -
Positive control
- Alpha Hemolysin Toxin; culture supernatant of USA300 strain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8B7 -
Isotype
IgG -
Research areas
Images
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Neutralising - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.
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![Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)](https://www.abcam.com/ps/products/190/ab190467/Images/ab190467-225602-anti-alpha-hemolysin-antibody-8b7-n-terminal-western-blot.jpg)
Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)All lanes : Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/ml
Lane 1 : alpha-emolysin at 0.1 µg
Lane 2 : Culture supernatant of USA300
Lane 3 : Negative control USA300 delta Hla strain
Predicted band size: 36 kDa
