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Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)

Key features and details

  • Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal
  • Suitable for: WB, Neutralising
  • Reacts with: Staphylococcus aureus
  • Isotype: IgG

Overview

  • Product name

    Anti-alpha-hemolysin antibody [8B7] - N-terminal

  • Description

    Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal

  • Host species

    Mouse

  • Specificity

    ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region.

  • Tested applications

    Suitable for: WB, Neutralisingmore details

  • Species reactivity

    Reacts with: Staphylococcus aureus

  • Immunogen

    Synthetic peptide corresponding to alpha-hemolysin (N terminal). Polypeptide sequence of Staphylococcus aureus alpha-hemolysin targeting the N terminus of the mature toxin.
    Database link: P09616

  • Positive control

    • Alpha Hemolysin Toxin; culture supernatant of USA300 strain

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Neutralising - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
    Neutralising - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)

    Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.

  • Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
    Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
    All lanes : Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/ml

    Lane 1 : alpha-emolysin at 0.1 µg
    Lane 2 : Culture supernatant of USA300
    Lane 3 : Negative control USA300 delta Hla strain

    Predicted band size: 36 kDa

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