Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)
![Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)](https://www.abcam.com/ps/products/247/ab247956/Images/ab247956-399012-anti-alpha-1-sodium-potassium-atpase-phospho-y10-antibody-epr2375-2-bsa-and-azide-free-western-blot.jpg "Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free
See all alpha 1 Sodium Potassium ATPase primary antibodies -
Description
Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects alpha 1 Sodium Potassium ATPase phosphorylated at Tyrosine 10.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab247956 is the carrier-free version of ab124677. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2375(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate, treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?This data was developed using ab124677, the same antibody clone in a different buffer formulation.
-
![Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)](https://www.abcam.com/ps/products/247/ab247956/Images/ab247956-2-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free (ab247956)