Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)
![Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)](https://www.abcam.com/ps/products/248/ab248459/Images/ab248459-396486-anti-aldh2-antibody-epr4494-western-blot-sw480-wt-hepg2-mcf7-sw480-aldh2-knockout.jpg "Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4494] to ALDH2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ALDH2 antibody [EPR4494] - BSA and Azide free
See all ALDH2 primary antibodies -
Description
Rabbit monoclonal [EPR4494] to ALDH2 - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and SW280 cell lysates.
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General notes
Ab248459 is the carrier-free version of ab133306. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4494 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ALDH2 knockout SW280 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab133306).
Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 56 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)](https://www.abcam.com/ps/products/248/ab248459/Images/ab248459-2-benefits-of-recombinant-antibodies.png)
Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)
