Anti-Alcam antibody [001] (ab279580)
![Anti-Alcam antibody [001] (ab279580)](https://www.abcam.com/ps/products/279/ab279580/Images/ab279580-1-CD166ALCAM-Antibody-ab279580-WB-SHSY5Y-Daudi-HuT78.jpg "Anti-Alcam antibody [001] (ab279580)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [001] to Alcam
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Alcam antibody [001]
See all Alcam primary antibodies -
Description
Rabbit monoclonal [001] to Alcam -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Rat Alcam. A DNA sequence encoding the rat ALCAM (Met1-Lys527) expressed, fused with a polyhistidine tag at the C-terminus.
Database link: O35112 -
Positive control
- WB: SH-SY5Y, Daudi and HuT78 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
001 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Alcam antibody (ab279580)All lanes : Anti-Alcam antibody [001] (ab279580) at 1/500 dilution
Lane 1 : SH-SY5Y Whole Cell Lysate
Lane 2 : Daudi Whole Cell Lysate
Lane 3 : HuT78 Whole Cell Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Observed band size: 100 kDa why is the actual band size different from the predicted?Developed using the Odyssey technique.
Performed under reducing conditions.