Anti-AKT1 antibody [RM252] (ab238477)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM252] to AKT1
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-AKT1 antibody [RM252]
See all AKT1 primary antibodies -
Description
Rabbit monoclonal [RM252] to AKT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: HEK-293 cell lysate. IHC-P: Human breast cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant -
Clonality
Monoclonal -
Clone number
RM252 -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for AKT1 using ab238477 at 1/1000 dilution in immunohistochemical analysis.
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Anti-AKT1 antibody [RM252] (ab238477) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Predicted band size: 56 kDa
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