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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT1 antibody [RM252] (ab238477)

Anti-AKT1 antibody [RM252] (ab238477)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM252] to AKT1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-AKT1 antibody [RM252]
    See all AKT1 primary antibodies
  • Description

    Rabbit monoclonal [RM252] to AKT1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HEK-293 cell lysate. IHC-P: Human breast cancer tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    RM252
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody [RM252] (ab238477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody [RM252] (ab238477)

    Formalin-fixed, paraffin-embedded human breast cancer tissue stained for AKT1 using ab238477 at 1/1000 dilution in immunohistochemical analysis.

  • Western blot - Anti-AKT1 antibody [RM252] (ab238477)
    Western blot - Anti-AKT1 antibody [RM252] (ab238477)
    Anti-AKT1 antibody [RM252] (ab238477) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Predicted band size: 56 kDa

  • Anti-AKT1 antibody [RM252] (ab238477)
    Anti-AKT1 antibody [RM252] (ab238477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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