Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGO4 antibody [EPR23799-22] (ab259821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23799-22] to AGO4
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGO4 antibody [EPR23799-22]
    See all AGO4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23799-22] to AGO4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, MCF7, THP-1, C2C12, NIH/3T3 and C6 whole cell lysates; Human liver and kidney tissue lysates; Mouse and rat kidney and spleen tissue lysates. IHC-P: Human colon carcinoma tissue; Mouse and rat kidney tissue. IP: THP-1 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Western blot - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    All lanes : Anti-AGO4 antibody [EPR23799-22] (ab259821) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human kidney tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat kidney tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 20146808). The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Immunoprecipitation - Anti-AGO4 antibody [EPR23799-22] (ab259821)

    AGO4 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte), whole cell lysate with ab259821 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259821 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 whole cell lysate 10 µg

    Lane 2: ab259821 IP in THP-1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259821 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Western blot - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    All lanes : Anti-AGO4 antibody [EPR23799-22] (ab259821) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 5 : C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse kidney. The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] (ab259821)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat kidney. The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-AGO4 antibody [EPR23799-22] (ab259821)
    Anti-AGO4 antibody [EPR23799-22] (ab259821)

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