Anti-Adenovirus Type 5 antibody (ab6982)
Key features and details
- Rabbit polyclonal to Adenovirus Type 5
- Suitable for: Neutralising, ELISA, WB, ICC/IF
- Isotype: IgG
Overview
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Product name
Anti-Adenovirus Type 5 antibody -
Description
Rabbit polyclonal to Adenovirus Type 5 -
Host species
Rabbit -
Tested applications
Suitable for: Neutralising, ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Purified Adenovirus type 5
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982) Image courtesy of an anonymous Abreview.ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted. -
Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)Additional information regarding Adenovirus type 5 capsid proteins. -
Western blot - Anti-Adenovirus Type 5 antibody (ab6982) Image courtesy of an anonymous AbreviewAll lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
All lanes : human hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocked with 5% milk
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982) Image courtesy of an anonymous Abreview.All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.