Anti-ADAM28 antibody - Aminoterminal end (ab28292)
Key features and details
- Rabbit polyclonal to ADAM28 - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM28 antibody - Aminoterminal end
See all ADAM28 primary antibodies -
Description
Rabbit polyclonal to ADAM28 - Aminoterminal end -
Host species
Rabbit -
Specificity
ab28292 recognises the Aminoterminal region of ADAM28. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to the Propeptide domain of human ADAM28.
Read Abcam's proprietary immunogen policy (Peptide available as ab41223.) -
Positive control
- Western blot: RD (rhabdomyosarcoma, human, embryonal, epithelial) cell lysates and A-431 (epidermoid carcinoma, human) cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADAM28 antibody - Aminoterminal end (ab28292)All lanes : Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 66 kDa could potentially be a cleaved form of ADAM28 due to the presence of an 18 amino acid signal peptide and a 180 amino acid propeptide. -
Western blot - Anti-ADAM28 antibody - Aminoterminal end (ab28292) Image courtesy of an anonymous Abreview.Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1/1000 dilution + whole cell lysate prepared from EL4 cells at 100000 cells
Secondary
HRP conjugated donkey anti-rabbit polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocked with 5% milk for 1 hour at 24°C.
