Anti-activated Notch1 antibody (ab52301)
Key features and details
- Rabbit polyclonal to activated Notch1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-activated Notch1 antibody
See all activated Notch1 primary antibodies -
Description
Rabbit polyclonal to activated Notch1 -
Host species
Rabbit -
Specificity
ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human activated Notch1 aa 1720-1770.
Sequence:A VQSETVEPPP PAQLHFMYVA AAAFVLLFFV GCGVLLSRKR RRQHGQLWFP
Database link: P46531 -
General notes
ab52301 is cleaved at Val 1744
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
PBS without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-activated Notch1 antibody (ab52301)All lanes : Anti-activated Notch1 antibody (ab52301) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour)
Lane 2 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour) with immunising peptide
Predicted band size: 272 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-activated Notch1 antibody (ab52301)Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.
