Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ACSS2 antibody [EPR8500] (ab133664)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8500] to ACSS2
Suitable for: ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-ACSS2 antibody [EPR8500]
See all ACSS2 primary antibodies
Description

Rabbit monoclonal [EPR8500] to ACSS2
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

corresponding to Human ACSS2 aa 100-200.
Database link: Q9NR19
Positive control
- WB: U-87 MG, HepG2, Caco 2 and fetal liver lysates, Mouse and Rat brain lysate; ICC/IF: Caco 2 and HepG2 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8500
Isotype

IgG
Research areas

Western blot - Anti-ACSS2 antibody [EPR8500] (ab133664)

All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Purified)

Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-ACSS2 antibody [EPR8500] (ab133664)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ACSS2 with purified ab133664 at 1:200 dilution (8.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-ACSS2 antibody [EPR8500] (ab133664)

All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Unpurified)

Lane 1 : U87 MG lysate
Lane 2 : HepG2 lysate
Lane 3 : Caco 2 lysate
Lane 4 : Fetal liver lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ACSS2 antibody [EPR8500] (ab133664)

Immunofluorescence analysis of ACSS2 in Caco 2 cells labelled with unpurified ab133664 at a 1/100 dilution.
Anti-ACSS2 antibody [EPR8500] (ab133664)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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