Anti-5HT3A receptor/5ht3 antibody (ab13897)
Key features and details
- Rabbit polyclonal to 5HT3A receptor/5ht3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-5HT3A receptor/5ht3 antibody
See all 5HT3A receptor/5ht3 primary antibodies -
Description
Rabbit polyclonal to 5HT3A receptor/5ht3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat 5HT3A receptor/5ht3. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
Database link: P35563 -
Positive control
- human brain lysate
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General notes
This product was previously labelled as 5HT3A receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)All lanes : Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/ml
Lane 1 : Human Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Predicted band size: 55 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)Western Blot of human brain lysate staining 5HT3A receptor with ab13897
