Anti-50S ribosomal protein L7/L12 antibody (ab225681)
Key features and details
- Rabbit polyclonal to 50S ribosomal protein L7/L12
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-50S ribosomal protein L7/L12 antibody -
Description
Rabbit polyclonal to 50S ribosomal protein L7/L12 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Staphylococcus aureus
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Immunogen
Recombinant full length protein (His-tag) corresponding to Staphylococcus aureus 50S ribosomal protein L7/L12 aa 1 to the C-terminus. (6xHis-SUMO tag at the N-terminal).
Database link: P66061 -
Positive control
- WB: Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-50S ribosomal protein L7/L12 antibody (ab225681)All lanes : Anti-50S ribosomal protein L7/L12 antibody (ab225681) at 1/500 dilution
Lane 1 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.08 µg
Lane 2 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.04 µg
Lane 3 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.025 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Predicted MWt including 6xHis-SUMO tag: 29 kDa
