Anti-14-3-3 Binding Domain (phospho S) antibody

Rabbit polyclonal to 14-3-3 Binding Domain (phospho S)

Rabbit

Ab14127 binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA ab14127 recognizes a wide range of peptides containing the 14-3-3 binding motif and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins.

Suitable for: WB, IP, IHC-P, ELISAmore details

Reacts with: Species independent

Synthetic phospho (Ser) 14-3-3-binding-motif peptides.

Liquid

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, BSA

Protein A purified

This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.

Polyclonal

IgG

• Neuroscience
• Neurology process
• Neural Signal Transduction

• Signal Transduction
• Adapters
• Cytoplasmic

• Stem Cells
• Signaling Pathways
• Wnt
• 14-3-3