Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free (ab247270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y62] to 14-3-3 alpha + beta - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free
    See all 14-3-3 alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [Y62] to 14-3-3 alpha + beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for 14-3-3 alpha + beta.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    Ab247270 is the carrier-free version of ab32560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247270 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free (ab247270)
    Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free (ab247270)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling 14-3-3 alpha + beta with purified ab32560 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32560).

  • Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free (ab247270)
    Anti-14-3-3 alpha + beta antibody [Y62] - BSA and Azide free (ab247270)

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