Annexin V-Biotin Assay Kit (ab273267)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Annexin V-Biotin Assay Kit -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Annexin V-Biotin Assay Kit (ab273267) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidin- or avidin-dye reagents, such as (strept)avidin-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β -gal, etc
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Tested applications
Suitable for: Flow Cyt, FMmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests 1X Binding Buffer 1 x 12.5ml Annexin V-Biotin 1 x 125µl Propidium Iodide 1 x 125µl