Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit (ab273373)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
Overview
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Product name
Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit
See all ACE2 kits -
Detection method
Fluorescent -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit (ab273373) can be used to screen for potent inhibitors of ACE2 activity, it utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an ACE2 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE2 inhibitors in a high-throughput format.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ACE2 Assay Buffer 1 x 25ml ACE2 Dilution Buffer 1 x 1.5ml ACE2 Enzyme 1 x 20µl ACE2 Inhibitor (0.5 mM) 1 x 5µl ACE2 Substrate 1 x 200µl -
Research areas
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
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Images
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Inhibition of ACE2 activity by ACE2 Inhibitor
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
IC50 = 33.0 ± 0.6 nM (n = 3). Assay was performed following the kit protocol