Aminohippuric acid Assay Kit (Colorimetric) (ab204725)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Plasma, Serum, Urine
Overview
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Product name
Aminohippuric acid Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma -
Assay type
Quantitative -
Product overview
Aminohippuric acid Assay Kit (ab204725) utilizes a 96-well format in which p-Aminohippuric acid (PAH) reacts with Dimethylaminocinnamaldehyde (DACA) which gives a strongly colored derivative at OD = 550 nm. The amounts of PAH in urine and plasma can easily be quantified with this product.
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Notes
p-Aminohippuric acid (PAH) is a derivative of hippuric acid and useful as a diagnostic agent for the measurement of renal plasma flow. About 20-30% is completely filtered by the glomerulus and not readsorbed by the tubules. The remainder which bypasses the glomerulus and enters the tubules is completely secreted. At low doses, PAH is almost completely removed with one-pass through the kidneys. Hence, the venous concentration of PAH is close to zero and PAH has been used to determine the effective renal plasma flow (eRPF) from the plasma. The venous concentration is usually
eRPF = ([PAH]U/[PAH]P) x Urine Volume
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests DACA solution 1 x 15ml PAH Standard (10 mg/ml) 1 x 100µl TCA (15 %) 1 x 15ml
Images
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Aminohippuric acid Assay Kit (Colorimetric) (ab204725)
Typical PAH Standard calibration curve