Alkaline Phosphatase chromogen (BCIP/TNBT) (ab7413)
Overview
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Product name
Alkaline Phosphatase chromogen (BCIP/TNBT)
See all Alkaline Phosphatase Chromogen reagents -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
General notes
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
Constituents: 0.02% BCIP, 0.04% TNBT -
Concentration information loading... -
Reagent notes
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures. -
Research areas