Alexa Fluor® 647 Anti-hnRNP K antibody [F45 P9 C7] (ab204455)
![Alexa Fluor® 647 Anti-hnRNP K antibody [F45 P9 C7] (ab204455)](https://www.abcam.com/ps/products/204/ab204455/Images/ab204455-248129-anti-hnrnp-k-antibody-f45-p9-c7-alexa-fluor-647-immunofluorescence.jpg "Alexa Fluor® 647 Anti-hnRNP K antibody [F45 P9 C7] (ab204455)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [F45 P9 C7] to hnRNP K
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-hnRNP K antibody [F45 P9 C7]
See all hnRNP K primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [F45 P9 C7] to hnRNP K -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
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Positive control
- ICC/IF: HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F45 P9 C7 -
Myeloma
Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-hnRNP K antibody [F45 P9 C7] (ab204455)
ab204455 staining hnRNP K in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204455 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
