Alexa Fluor® 647 Anti-CD86 antibody [OX48] (ab256271)
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [OX48] to CD86
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-CD86 antibody [OX48]
See all CD86 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [OX48] to CD86 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: LPS activated Lewis rat splenocytes.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
OX48 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Lewis rat splenocytes treated with 10 µg/ml LPS for 3 days were stained with ab256271 (right) or mouse IgG1κ Alexa Fluor® 647 isotype (left). 10 µg/ml LPS for 3 days were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256271) or mouse IgG1κ Alexa Fluor® 647 isotype (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD45R.
Acquisition of >30,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on live lymphocytes.