7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of non-viable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Exitation = 540 nm / emission = 645 nm.