6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
Key features and details
- Detection method: Colorimetric
- Sample type: Adherent cells, Suspension cells, Tissue Homogenate
Overview
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Product name
6-Phosphogluconate Dehydrogenase Assay Kit -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells, Tissue Homogenate -
Product overview
6-Phosphogluconate Dehydrogenase Assay Kit (ab241016) provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples.
In this assay, 6-PGD converts 6-phosphogluconate into an intermediate, generating NADPH, which subsequently reduces a colorless probe into a strongly colored product detectable by absorbance at 460 nm.
The assay is high- throughput adaptable and can detect less than 0.05 mU of 6-PGD activity.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 6-PGD Assay Buffer 1 x 25ml 6-PGD Developer 1 vial 6-PGD Positive Control 1 vial 6-PGD Substrate 1 vial NADPH Standard 1 vial -
Research areas
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Function
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3. -
Sequence similarities
Belongs to the 6-phosphogluconate dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 0610042A05Rik
- 6 phosphogluconate dehydrogenase, decarboxylating
- 6-phosphogluconate dehydrogenase
see all
Images
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NADPH standard curve.NADPH standard curve.
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Reaction kinetics of 6-Phosphogluconate dehydrogenase activity.Reaction kinetics of 6-Phosphogluconate dehydrogenase activity in positive control, rat liver (66 µg protein) and rat spleen (56 µg protein) using appropriate background controls.
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6-Phosphogluconate dehydrogenase specific activity.6-Phosphogluconate dehydrogenase specific activity calculated in rat liver and spleen tissue lysates. Assays were performed following the kit protocol.