25x TBS (pH 7.4) (ab64248)
Overview
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Product name
25x TBS (pH 7.4)
See all TBS reagents -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Tris Buffered Saline (TBS) wash buffer ab64248 for use during immunolabeling techniques such as IHC.
In order to provide this product at 25x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution:
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 7
Constituents: 22% Sodium chloride, 20.8% Tris HCl
Buffer 25x concentrated. Made from Tris base and HCl. -
Concentration information loading... -
Research areas
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Relevance
TBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies. -
Alternative names
- Tris buffered saline