10kD Spin Column (ab93349)
Overview
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Product name
10kD Spin Column -
Product overview
The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.
They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL.
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Notes
If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 25 units Concentrator cup: Polycarbonate 25 units Filtrate vessel: Polypropylene 25 units Membrane: 10,000 MWCO Polyethersulfone 25 units -
Research areas
Images
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- 10kD Spin Column (ab93349)
Representative 10kD Spin Column (ab93349).