Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cell Biology Other Antibodies

Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

Price and availability

335 587 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21912-3] to YTHDF3
  • Suitable for: Flow Cyt, IP, WB, IHC-Fr, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

You may also be interested in

Product image
Anti-SPACA4 antibody (ab122758)
Product image
Recombinant Human LSM12 protein (ab139209)
Product image
Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)
Product image
Human TOR3A knockout HeLa cell line (ab265113)

Overview

  • Product name

    Anti-YTHDF3 antibody [EPR21912-3]
    See all YTHDF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR21912-3] to YTHDF3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type mESC whole cell lysate. GST-tagged human YTHDF3 recombinant protein. Fresh HT-1080, HeLa, NIH/3T3, PC-12 whole cell lysate. IHC-P: Mouse and rat cerebrum tissue. Human colon tissue. IHC-Fr: Mouse and rat cerebrum tissue. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21912-3
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    All lanes : Anti-YTHDF3 antibody [EPR21912-3] (ab220161) at 1/1000 dilution

    Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate
    Lane 2 : YTHDF3 knockout mESC whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    The wild-type and YTHDF3 knockout cell lysates were kindly provided by an anonymous collaborator.

    ab220161 was shown to specifically react with YTHDF3 in wild-type mESC cells as signal was lost in YTHDF3 knockout cells. Wild-type and YTHDF3 knockout samples were subjected to SDS-PAGE. ab220161 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling YTHDF3 with ab220161 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human colon (PMID:29103884, 28250115) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunohistochemistry (Frozen sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling YTHDF3 with ab220161 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic and nuclear staining in mouse cerebrum (PMID:29103884, 28250115) is observed. Counterstained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Flow Cytometry - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Flow Cytometry - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling YTHDF3 with ab220161 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunoprecipitation - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    YTHDF3 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab220161 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220161 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab220161 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220161 in HeLa whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

  • Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    All lanes : Anti-YTHDF3 antibody [EPR21912-3] (ab220161) at 1/1000 dilution

    Lane 1 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate (fresh lysate)
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (fresh lysate)
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (fresh lysate)
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (fresh lysate)
    Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa



    Lysate should be made freshly and used in WB immediately to minimize protein degradation (lane1-4). Lane 5-7 are the lysates from same cell lines but have experienced freeze-thaw cycles.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 37 secs; Lanes 5-7: 92 secs.

  • Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Western blot - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    All lanes : Anti-YTHDF3 antibody [EPR21912-3] (ab220161) at 1/5000 dilution

    Lane 1 : GST-tagged human YTHDF1 recombinant protein 20 ng
    Lane 2 : GST-tagged human YTHDF2 recombinant protein 20 ng
    Lane 3 : GST-tagged human YTHDF3 recombinant protein 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa


    Exposure time: 10 seconds


    The YTHDF recombinant proteins were kindly provided by an anonymous collaborator.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunohistochemistry (Frozen sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling YTHDF3 with ab220161 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic and nuclear staining in rat cerebrum (PMID:29103884, 28250115) is observed. Counterstained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling YTHDF3 with ab220161 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on neurons of rat cerebrum (PMID:29103884, 28250115) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling YTHDF3 with ab220161 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on neurons of mouse cerebrum (PMID:29103884, 28250115) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-YTHDF3 antibody [EPR21912-3] (ab220161)
    Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-YTHDF3 antibody [EPR21912-3] (ab220161)

  •  
  • Product image

    Anti-YTHDF3 antibody [EPR21912-3] - BSA and Azide free (ab255267)

    Applications: Flow Cyt, IHC-Fr, IHC-P, IP, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.