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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

Price and availability

377 662 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [RV202] to Vimentin - Cytoskeleton Marker
  • Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Pig, Zebrafish
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Vimentin antibody [RV202] - Cytoskeleton Marker
    See all Vimentin primary antibodies
  • Description

    Mouse monoclonal [RV202] to Vimentin - Cytoskeleton Marker
  • Host species

    Mouse
  • Specificity

    This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    Human
    IHC-Fr
    Pig
    Zebrafish
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Bovine Vimentin. RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a vimentin extract of bovine lens.
    Database link: P08670

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Vimentin protein
    • IHC-Fr: Human lung, Ed18 rat, pig colon and zebrafish embryo. ICC/IF: Human colon, rat brain, SK-N-SH, human pancreatic adenocarcinoma cells and HAP1 cells. WB: NIH-3T3 and normal human dermal fibroblasts cell extracts. IHC-P: Human placenta, human small intestine, human spleen, human tonsillar lymphoma, human colon tissue, human fetal kidney tissue, dog soft tissue sarcoma tissue.
  • General notes

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    RV202
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    ab8978 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8978 at 1μg/ml and ab202272 (Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 594)) at 1/250 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with ab150117 (Goat secondary antibody to Mouse IgG (Alexa Fluor® 488)) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Western blot - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    All lanes : Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Normal human dermal fibroblasts cell extracts.

    Predicted band size: 53 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human placenta labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

  • Flow Cytometry - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Flow Cytometry - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Overlay histogram showing HeLa cells stained with ab8978 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8978, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This anti-Vimentin antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) Rauschert, I. et al PLoS One. 2017 Apr 19;12(4):e0175953. doi: 10.1371/journal.pone.0175953. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Silencing of Lamin A/C in unmethylated neuroblastoma cells induces changes in different cytoskeletal components

    Immunofluorescence staining showing changes in SK-N-SH-lamin-A/C-shRNA compared with SK-N-SH-scramble-shRNA in (A) β-actin filaments, (B) F-actin filaments. (C) Vimentin filaments, and (D) α-tubulin. Lamin A/C is shown in green; β-actin, F-actin, vimentin, and α-tubulin are shown in red.DNA is stained in blue (DAPI). Scale bar, 10μM.

    Vimentin is detected using ab8978 at 1/100 dilution. Neuroblastoma cells were fixed with 4% paraformaldehyde and permeabilized using 0.5% Triton X-100.

    (From Figure 5C of Rauschert et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human small intestine labeling Vimentin with ab8978; showing positive staining in connective tissue cells and no reactivity in epithelial cells.

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human spleen labeling Vimentin with ab8978, showing positive staining in connective tissue cells and lymphoid cells.

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Paraffin-embedded human colon tissue stained for Vimentin using ab8978 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of an anonymous abreview.

    ab8978 staining Vimentin in human fetal kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with CAS-Block for 1 hour at 25°C; antigen retrieval was by heat mediation using OmniPrep (pH 9). Samples were incubated with primary antibody (1/500) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Donkey polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunofluorescence staining images of 9 day old zebrafish embryos. ab8978 reacts with in connective tissue cells and bloodvessels. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of an Abreview submitted by Dr J. Chai

    ab8978 staining Vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized in 0.1% Triton and blocked with 0.25% serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 2 hours at 28°C. ab6785 Goat polyclonal anti-Mouse IgG - H&L (FITC) (1/800) was used as the secondary antibody. Nuclei were counterstained with propidium iodide.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of an anonymous abreview.

    ab8978 staining Vimentin in dog soft tissue sarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. Samples were incubated with primary antibody (1/100 in TBS) for 18 hours at 20°C. A Alexa Fluor® 647-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) Image courtesy of an AbReview submitted by Dr Ruma Raha-Chowdhury

    IHC-Fr image of Ed18 rat stained with ab8978. Fresh frozen sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3% triton X100  for 1h to permeabilise the tissues and block non-specific protein-protein interactions. The sectons were then incubated with the ab8978 (1µg/ml) and ferroportin overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) donkey anti-mouse at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Vimentin expressed in the gut muscles.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human tonsillar lymphoma labeling Vimentin with ab8978.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

    IHC-FoFr image of vimentin staining on rat injured cortical sections using ab8978 (1:500). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1 hour at 24°C. ab8978 was diluted 1:500 and incubated with sections for 24 hours using 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of an Abreview submitted by Jennifer Dembinski

    ab8978 staining vimentin in human pancreatic adenocarcinoma cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.2% Triton X prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 21°C. Alexa fluor® 488 mouse polyclonal to mouse Ig, diluted 1/300 was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) This image is courtesy of an anonymous abreview.

    ab8978 staining Vimentin in human lung tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 5% commercially available blocking agent was performed at 370C  for 15 minutes. The sample was incubated with primary antibody (1/250) at 370C for 1 hour. A HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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