Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Immune System Diseases Antiviral Signaling HIV

Anti-TDP43 antibody [EPR18554] (ab190963)

Price and availability

317 504 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-TDP43 antibody [EPR18554] (ab190963)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18554] to TDP43
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Human, Zebrafish

You may also be interested in

Anti-HIV1 p17 antibody [32/5.8.42] (ab9064)
Anti-HIV1 p24 antibody [39/5.1.23] (ab9070)
Product image
Anti-HIV1 tat antibody [N3] (ab63957)
Product image
Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)

Overview

  • Product name

    Anti-TDP43 antibody [EPR18554]
    See all TDP43 primary antibodies
  • Description

    Rabbit monoclonal [EPR18554] to TDP43
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Zebrafish
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293 and K562 whole cell lysates; Human fetal brain and fetal heart lysates; Zebrafish head lysate; Untreated HeLa whole cell lysates and HeLa whole cell lysates treated with 1µM staurosporine for 4 hours, 50 µM Z-VAD-FMK for 4 hours and 1µM staurosporine and 50 µM Z-VAD-FMK for 4 hours. IHC-P: Human pancreas and endometrium carcinoma tissues; Mouse and rat liver tissues. ICC/IF: HeLa and K562 cells. Flow Cyt: K562 cells. IP: HeLa whole cell lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18554
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] (ab190963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] (ab190963)

    Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling TDP43 with ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on tumor cells of the endometrium carcinoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: TDP43 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab190963 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab190963 was shown to specifically react with TDP43 in wild type cells as signal was lost in TDP43 knockout cells. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. ab190963 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] (ab190963)
    Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] (ab190963)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TDP43 with ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab190963 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Flow Cytometry - Anti-TDP43 antibody [EPR18554] (ab190963)
    Flow Cytometry - Anti-TDP43 antibody [EPR18554] (ab190963)

    Flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling TDP43 with ab190963 at 1/1000 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Anti-TDP43 antibody [EPR18554] (ab190963) at 1/2000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    All lanes : Anti-TDP43 antibody [EPR18554] (ab190963) at 1/10000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350).

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    All lanes : Anti-TDP43 antibody [EPR18554] (ab190963) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350).

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Anti-TDP43 antibody [EPR18554] (ab190963) at 1/1000 dilution + Zebrafish head lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350).

  • Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    Western blot - Anti-TDP43 antibody [EPR18554] (ab190963)
    All lanes : Anti-TDP43 antibody [EPR18554] (ab190963) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 1µM staurosporine for 4 hours whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 50 µM Z-VAD-FMK for 4 hours whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 1µM staurosporine and 50 µM Z-VAD-FMK for 4 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350), and can be induced by staurosporine.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] (ab190963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] (ab190963)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TDP43 with ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on normal Human pancreas is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] (ab190963)
    Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] (ab190963)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling TDP43 with ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on K562 cell line.

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab190963 at 1/2000 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-TDP43 antibody [EPR18554] (ab190963)
    Immunoprecipitation - Anti-TDP43 antibody [EPR18554] (ab190963)

    TDP43 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab190963 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab190963 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10µg (Input).

    Lane 2: ab190963 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab190963 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350).

  • Anti-TDP43 antibody [EPR18554] (ab190963)
    Anti-TDP43 antibody [EPR18554] (ab190963)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TDP43 antibody [EPR18554] (ab190963)

  •  
  • Product image

    Anti-TDP43 antibody [EPR5811] (ab133547)

    Applications: Flow Cyt, ICC, IHC-P, WB

  •  
  • Product image

    Anti-TDP43 antibody [DB9] (ab254166)

    Applications: ELISA, IHC-P, WB

  •  
  • Product image

    Anti-TDP43 antibody [EPR5810] - BSA and Azide free (ab185133)

    Applications: Flow Cyt, ICC, IHC-Fr, IHC-P, WB

  •  
  • Product image

    Anti-TDP43 antibody (ab80608)

    Applications: WB

  •  
  • Product image

    Alexa Fluor® 568 Anti-TDP43 antibody [EPR5811] (ab208544)

    Applications: ICC/IF

  •  
  • Product image

    Alexa Fluor® 488 Anti-TDP43 antibody [EPR18554] (ab237270)

    Applications: ICC/IF

  •  
  • Product image

    Anti-TDP43 antibody (ab225710)

    Applications: IHC-P, IP, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

    Applications: Flow Cyt, ICC/IF

  •  
  • Product image

    Anti-TDP43 antibody [3H8] (ab104223)

    Applications: Flow Cyt, ICC/IF, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.