Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2588(2)] to SOCS2 - BSA and Azide free
  • Suitable for: ICC, IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free
    See all SOCS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2588(2)] to SOCS2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT and K-562 whole cell lysates; IP: K-562 whole cell lysate; Flow Cyt: HeLa cells; ICC: MCF7 cells.

  • General notes

    Ab247806 is the carrier-free version of ab109245. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247806 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    Western blot - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    All lanes : Anti-SOCS2 antibody [EPR2588(2)] (ab109245) at 1/2000 dilution (purified)

    Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

  • Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

  • Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/150 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

  • Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)

    Purified ab109245 at 1/70 dilution (2 µg) immunoprecipitating SOCS2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab109245 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109245 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 22 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109245).

  • Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)
    Anti-SOCS2 antibody [EPR2588(2)] - BSA and Azide free (ab247806)

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