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Microbiology Interspecies Interaction Host Virus Interaction

Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

Price and availability

2 142 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL33] to SLC1A5/ASCT2
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-SLC1A5/ASCT2 antibody [CAL33]
    See all SLC1A5/ASCT2 primary antibodies
  • Description

    Rabbit monoclonal [CAL33] to SLC1A5/ASCT2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon carcinoma and lung carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. ICC/IF: Jurkat and HeLa cells. Flow Cyt: Jurkat and HeLa cells. IP: Jurkat and HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SLC1A5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purity is greater than 99%.
  • Clonality

    Monoclonal
  • Clone number

    CAL33
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human colon carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human lung carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SLC1A5/ASCT2 using ab237704 at 0.5 µg/ml in immunohistochemical analysis.

  • Western blot - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Western blot - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    All lanes : Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Immunofluorescent analysis of 100% methanol fixed Jurkat (human T cell leukemia T lymphocyte) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HeLa cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

  • Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

    SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Jurkat whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in Jurkat whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in Jurkat whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

  • Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)
    Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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