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Microbiology Interspecies Interaction Host Virus Interaction

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Price and availability

361 000 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
    See all Scavenging Receptor SR-BI primary antibodies
  • Description

    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WTV0

  • Positive control

    • WB: Mouse liver tissue lysate. IHC-P: Human liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1556Y
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Human liver whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 lysate
    Lane 3 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Observed band size: 80 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

     

  • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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