Anti-RanBP1 antibody [EPR6796(B)] (ab133550)
![Anti-RanBP1 antibody [EPR6796(B)] (ab133550)](https://www.abcam.com/ps/products/133/ab133550/Images/ab133550-410220-anti-ranbp1-antibody-epr6796-western-blot-human-knockout-lysate.jpg "Anti-RanBP1 antibody [EPR6796(B)] (ab133550)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6796(B)] to RanBP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RanBP1 antibody [EPR6796(B)]
See all RanBP1 primary antibodies -
Description
Rabbit monoclonal [EPR6796(B)] to RanBP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human RanBP1 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2 and HeLa cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6796(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RanBP1 antibody [EPR6796(B)] (ab133550)All lanes : Anti-RanBP1 antibody [EPR6796(B)] (ab133550) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : RANBP1 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133550 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133550 Anti-RanBP1 antibody [EPR6796(B)] was shown to specifically react with RanBP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266202 (knockout cell lysate ab258168) was used. Wild-type and RanBP1 knockout samples were subjected to SDS-PAGE. ab133550 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-RanBP1 antibody [EPR6796(B)] (ab133550)](https://www.abcam.com/ps/products/133/ab133550/Images/RanBP1-Primary-antibodies-ab133550-1.jpg)
Western blot - Anti-RanBP1 antibody [EPR6796(B)] (ab133550)All lanes : Anti-RanBP1 antibody [EPR6796(B)] (ab133550) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
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![Anti-RanBP1 antibody [EPR6796(B)] (ab133550)](https://www.abcam.com/ps/products/133/ab133550/Images/ab133550-3-benefits-of-recombinant-antibodies.png)
Anti-RanBP1 antibody [EPR6796(B)] (ab133550)
