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Kits/ Lysates/ Other Tools and Reagents Western Blot Tools and Reagents HRP

Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Goat monoclonal [RMG01] to Rabbit IgG Fab region
  • Suitable for: WB, ELISA
  • Reacts with: Rabbit

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Overview

  • Product name

    Anti-Rabbit IgG Fab region antibody [RMG01]
  • Description

    Goat monoclonal [RMG01] to Rabbit IgG Fab region
  • Host species

    Goat
  • Specificity

    ab190495 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Rabbit
    Does not react with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab190495 purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMG01
  • Isotype

    IgG
  • Research areas

    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

Images

  • ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

    ELISA analysis of different species’ IgG shows ab190495 reacted to the Fab region of Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG as the secondary antibody.

  • ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

    Titer ELISA analysis of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG was used as the secondary antibody.

  • Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    All lanes : Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495) at 0.2 µg/ml

    Lane 1 : Non-reduced Rabbit IgG
    Lane 2 : Reduced Rabbit IgG

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 150 kDa

  • Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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