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Neuroscience Cell Type Marker Neuron marker Soma marker

Anti-Islet 1 antibody [EPR10362] (ab178400)

Price and availability

338 644 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Islet 1 antibody [EPR10362] (ab178400)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10362] to Islet 1
  • Suitable for: IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Islet 1 antibody [EPR10362]
    See all Islet 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10362] to Islet 1
  • Host species

    Rabbit
  • Specificity

    In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human Islet 1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P61371

  • Positive control

    • WB: HeLa, K562, A431, A375, Jurkat and SH-SY5Y cell lysates. IHC-P: Human gastric neuroendocrine carcinoma, pancreas and small cell lung carcinoma tissues. IP: SH-SY5Y cell lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10362
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Islet 1 with purified ab178400 at 1/6000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    All lanes : Anti-Islet 1 antibody [EPR10362] (ab178400) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Islet 1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 39 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab178400 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Unpurified ab178400 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. Ab178400 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    All lanes : Anti-Islet 1 antibody [EPR10362] (ab178400) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)

    ab178400 (purified ) at 1/20 dilution (1ug) immunoprecipitating Islet 1 in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab178400 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178400 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Islet 1 with ab178400  followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human pancreatic islet. The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Anti-Islet 1 antibody [EPR10362] (ab178400) at 1/10000 dilution ((unpurified)) + A375 (HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, human gastric neuroendocrine carcinoma tissue labeling Islet 1 with unpurified ab178400 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)

    Islet 1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg with unpurifed ab178400 at 1:50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using unpurified ab178400 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg

    Lane 2: ab178400 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab178400 in SH-SY5Y whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second

  • Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Western blot - Anti-Islet 1 antibody [EPR10362] (ab178400)
    All lanes : Anti-Islet 1 antibody [EPR10362] (ab178400) at 1/1000 dilution ((unpurified))

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, human small cell lung carcinoma tissue labeling Islet 1 with unpurified ab178400 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)
    Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] (ab178400)

    Western blot analysis on Immunoprecipitation pellet from either Lane 1) SH-SY5Y cell lysate, or Lane 2) 1xPBS (negative control); showing Islet 1, using unpurifed ab178400 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-Islet 1 antibody [EPR10362] (ab178400)
    Anti-Islet 1 antibody [EPR10362] (ab178400)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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