Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Microbiology Interspecies Interaction Host Virus Interaction

Anti-HMGA1 antibody [EPR7839] (ab129153)

Price and availability

337 557 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-HMGA1 antibody [EPR7839] (ab129153)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7839] to HMGA1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Human ZC3HAV1 (Zinc finger antiviral protein) knockout A549 cell line (ab266952)
Product image
Alexa Fluor® 488 Anti-CRTC3 antibody [EPR3440] (ab206248)
Product image
Recombinant Human UBR4/p600 protein (ab153218)
Product image
STAT1 (pY701) ELISA Kit (ab126456)

Overview

  • Product name

    Anti-HMGA1 antibody [EPR7839]
    See all HMGA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7839] to HMGA1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human HMGA1 (N terminal). The exact sequence is proprietary.
    Database link: P17096

  • Positive control

    • WB: SK-OV-3, Caco-2, BxPC-3 and HepG2 whole cell lysate (ab7900), rat kidney and mouse brain tissue lysates. IHC-P: Human testis and transitional cell carcinoma of bladder tissues. ICC/IF: HeLa and BxPC-3 cells. Flow Cyt: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7839
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) (purified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : SK-OV-3 cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Immunocytochemistry/Immunofluorescence analysis of BxPC-3 cells labelling HMGA1 with unpurified ab129153 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling HMGA1 with purified ab129153 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)
    All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (unpurified)

    Lane 1 : SK-OV-3 cell lysate
    Lane 2 : Caco-2 cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling HMGA1 with unpurified ab129153 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HMGA1 with purified ab129153 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Flow cytometry analysis of HepG2 cells labelling HMGA1 with purified ab129153 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)
    Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)

    Overlay histogram showing HepG2 cells stained with unpurified ab129153 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129153, 1/870 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-HMGA1 antibody [EPR7839] (ab129153)
    Anti-HMGA1 antibody [EPR7839] (ab129153)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HMGA1 antibody [EPR7839] (ab129153)

  •  
  • Product image

    Alexa Fluor® 488 Anti-HMGA1 antibody [EPR7839] (ab204667)

    Applications: ICC/IF

  •  
  • Product image

    Anti-HMGA1 antibody [EPR16649] (ab202070)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-HMGA1 antibody [EPR22421-101] - ChIP Grade (ab252930)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-HMGA1 antibody (ab230384)

    Applications: IHC-P

  •  
  • Product image

    Anti-HMGA1 antibody (ab168260)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-HMGA1 antibody - N-terminal (ab226850)

    Applications: ICC/IF, WB

  •  
  • Product image

    PE Anti-HMGA1 antibody [EPR7839] (ab209761)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-HMGA1 antibody [EPR7839] - BSA and Azide free (ab226112)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.