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Anti-Histone H4 antibody [RM212] (ab222763)

Anti-Histone H4 antibody [RM212] (ab222763)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM212] to Histone H4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-Histone H4 antibody [RM212]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [RM212] to Histone H4
  • Host species

    Rabbit
  • Specificity

    ab222763 reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (C terminal).
    Database link: P62805

  • Positive control

    • WB: Recombinant Histone H4 protein; A-375, HEK-293, HeLa and SK-MEL-2 whole cell lysates. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab222763 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM212
  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [RM212] (ab222763)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [RM212] (ab222763)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (red) using ab222763 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).

  • Western blot - Anti-Histone H4 antibody [RM212] (ab222763)
    Western blot - Anti-Histone H4 antibody [RM212] (ab222763)
    All lanes : Anti-Histone H4 antibody [RM212] (ab222763) at 0.2 µg/ml

    Lane 1 : A-375 (human malignant melanoma cell line) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : SK-MEL-2 whole cell lysate

    Predicted band size: 11 kDa

  • Western blot - Anti-Histone H4 antibody [RM212] (ab222763)
    Western blot - Anti-Histone H4 antibody [RM212] (ab222763)
    All lanes : Anti-Histone H4 antibody [RM212] (ab222763) at 0.2 µg/ml

    Lane 1 : Recombinant Histone H2A protein
    Lane 2 : Recombinant Histone H2B protein
    Lane 3 : Recombinant Histone H3.1 protein
    Lane 4 : Recombinant Histone H3.3 protein
    Lane 5 : Recombinant Histone H4 protein

    Predicted band size: 11 kDa



    ab222763 is specific to Histone H4.

  • Anti-Histone H4 antibody [RM212] (ab222763)
    Anti-Histone H4 antibody [RM212] (ab222763)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Histone H4 antibody [RM212] (ab222763)

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    Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

    Applications: ICC/IF, IHC-P, PepArr, WB

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    Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Applications: ChIP, ICC/IF, IHC-P, PepArr, WB

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    Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Applications: Flow Cyt, ICC/IF, IHC-P, PepArr, WB

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    Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)

    Applications: ChIP, Dot, IHC-P, WB

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    Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)

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