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Microbiology Interspecies Interaction Host Virus Interaction

Anti-GMEB1 antibody [EPR23056-62] (ab240646)

Price and availability

306 850 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-GMEB1 antibody [EPR23056-62] (ab240646)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23056-62] to GMEB1
  • Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-GMEB1 antibody [EPR23056-62]
    See all GMEB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR23056-62] to GMEB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with mouse GMEB1, HeLa, A549, RAW264.7 and PC-12 lysates. IHC-P: Human colon, Human spleen, Human tonsil, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt: HeLa and HEK-293T cells. IP: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23056-62
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Corticoid
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Western blot - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Western blot - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    All lanes : Anti-GMEB1 antibody [EPR23056-62] (ab240646) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 63 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 26 seconds; Lane 2: 125 seconds; Lane 3: 169 seconds; Lane 4: 3 minutes.

    The weak lower bands below 75kDa could be degraded GMEB1 fragments (PMID:10386584).

  • Western blot - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Western blot - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    All lanes : Anti-GMEB1 antibody [EPR23056-62] (ab240646) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with mouse GMEB1 (WT)(aa1-562) expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The weak lower bands below 75kDa could be degraded GMEB1 fragments (PMID:10386584).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GMEB1 with ab240646 at 1/2000 dilution (0.288 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat spleen. The section was incubated with ab240646 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GMEB1 with ab240646 at 1/1000 dilution (0.576 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse spleen (PMID: 10692587) is observed. The section was incubated with ab240646 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GMEB1 with ab240646 at 1/1000 dilution (0.576 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human tonsil (PMID: 16497673) is observed. The section was incubated with ab240646 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling GMEB1 with ab240646 at 1/1000 dilution (0.576 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human spleen (PMID: 16497673) is observed. The section was incubated with ab240646 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GMEB1 with ab240646 at 1/1000 dilution (0.576 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human colon (PMID: 16497673) is observed. The section was incubated with ab240646 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunoprecipitation - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    GMEB1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240646 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240646 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab240646 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240646 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

     

  • Flow Cytometry - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Flow Cytometry - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with mouse GMEB1 (WT)(aa1-562) expression vector containing a myc-His-tag® cells labelling GMEB1 with ab240646 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab240646 (Right). Then stained with anti-myc tag conjugated to PE.

  • Flow Cytometry - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Flow Cytometry - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling GMEB1 with ab240646 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunocytochemistry/ Immunofluorescence - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling GMEB1 with ab240646 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in Neuro-2a cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is  ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Immunocytochemistry/ Immunofluorescence - Anti-GMEB1 antibody [EPR23056-62] (ab240646)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling GMEB1 with ab240646 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000  dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Anti-GMEB1 antibody [EPR23056-62] (ab240646)
    Anti-GMEB1 antibody [EPR23056-62] (ab240646)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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