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Developmental Biology Lineage specification Mesoderm

Anti-Glypican 3 antibody [EPR10641] (ab174851)

Price and availability

290 806 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Glypican 3 antibody [EPR10641] (ab174851)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10641] to Glypican 3
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Glypican 3 antibody [EPR10641]
    See all Glypican 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10641] to Glypican 3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human Glypican 3. The exact sequence is proprietary.
    Database link: P51654

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal liver lysate; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10641
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Signal Transduction
    • Cytoskeleton / ECM
    • HSPGs
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Images

  • Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)
    Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.

    ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. Ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] (ab174851)
    Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] (ab174851)

    Flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red)  or a rabbit IgG negative (green).

  • Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)
    Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)
    All lanes : Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa

  • Anti-Glypican 3 antibody [EPR10641] (ab174851)
    Anti-Glypican 3 antibody [EPR10641] (ab174851)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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