Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Antibodies gfp

Anti-GFP antibody (ab290)

Price and availability

381 998 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-GFP antibody (ab290)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GFP
  • Suitable for: ELISA, IHC-FrFl, Electron Microscopy, IHC-FoFr, ICC, IHC-P, IHC-Fr, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG

You may also be interested in

Anti-GFP antibody (ab92456)
Product image
Anti-GFP antibody (ab5449)
Product image
HRP Anti-GFP antibody [E385] (ab190584)
Product image
FITC Anti-GFP antibody (ab6662)

Overview

  • Product name

    Anti-GFP antibody
    See all GFP primary antibodies
  • Description

    Rabbit polyclonal to GFP
  • Host species

    Rabbit
  • Specificity

    Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

    This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP and EGFP.

     

  • Tested Applications & Species

    Application Species
    ICC
    Species independent
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria.
    Database link: P42212

  • Positive control

    • The Recombinant A. victoria GFP protein (ab84191), any other purified recombinant GFP, any cell line confirmed to overexpress GFP. ICC: NIH3T3, U2OS and glandular stomach cells. IHC: Mouse brain and dog heart tissue. WB: Sample: COS7 and LNCaP whole cell lysate - transfected with GFP-Eml4.
  • General notes

    The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

    Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products).

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 1.25% Sodium chloride
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    This antibody is provided as whole antiserum. It is not possible to determine the exact antibody concentration, since whole serum contains many other host serum proteins besides the antibody of interest.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290) Image from Yang C et al., PLoS One. 2013;8(11):e79615. Fig 2.; doi:10.1371/journal.pone.0079615. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Bone marrow-derived infiltrating cells in the stromal tissue of gastric intraepithelial tumor traced by GFP direct fluorescence.

    (A) Normal tissues of the glandular stomach of a regular GFP(−) control mouse. (B) Normal tissues of the glandular stomach of a GFP(+) transgenic control mouse; (C, E, D, F) An induced gastric intraepithelial neoplasia (GIN) in a bone marrow transplanted mouse. GFP(+) BMDCs tracked with direct fluorescence localized in the GIN stromal tissue are shown in C and E. The same GIN lesion slide stained by H&E after the fluorescence observation are shown in D and F. DAPI (A–C and E) and hematoxylin (D and F) are used to visualize nuclei, respectively. Locations of the images C and D in the images E and F, and the image E in the image F are marked in the corresponding color. The gastric glands and stromal cells are also labeled.

  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)
    Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290) This image is courtesy of an Abreview submitted by Judith Kranz

    Immunohistochemistry (Free Floating) analysis of mouse brain tissue sections labelling GFP with ab290. Tissue was fixed with 4% PFA, frozen 30 µm sections were blocked for 1 hour at room temperature with 10% normal goat serum + donkey anti-mouse IgG Fab fragments (0.1 mg/ml). Sections were incubated with the primary antibody at a dilution of 1/1000 in TBS + 0.25% Triton-X for 16 hours at 4°C. A Cy2®-conjugated donkey anti-rabbit IgG (H+L) at a dilution of 1/200 was used as the secondary antibody.

    Image shows anti-NeuN (red), DAPI (blue), and anti-GFP staining of GFP-cre (green, yellow with NeuN colocalization).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290) This image is courtesy of an anonymous Abreview

    ab290 staining dog hearts (Adv-GFP injection) tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citric acid (Ph6.0, 0.05% Tween20) prior to blocking with 10% serum for 30 mins at 37°C.  The primary antibody was diluted 1/1000 in PBS and incubated with the sample for 1 hour at 25°C.  A HRP conjugated secondary like  Goat Anti-Rabbit IgG H&L (HRP) (ab205718) was used.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    Immunofluorescence images showing similar localization of Yes-GFP (first 10 aa's of Yes PTK fused to the N-terminus of GFP) to full length Yes PTK. A: Distribution of Yes detected using mouse anti-Yes Ab followed by Texas Red-conjugated anti-mouse Ab. B: Chimeric GFP's detected using rabbit anti-GFP Ab (Abcam ab290) followed by FITC-conjugated anti-rabbit Ab.

    Image kindly provided by L.G. Berthiaume. Taken from J. McCabe and L.G. Berthiaume, Functional Roles for Fatty Acylated Amino-terminal Domains in Subcellular Localization, Molecular Biology of the Cell 10:3771-3786, 1999

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290) This image is courtesy of an Abreview submitted by William Hung

    ab290 immunoprecipitating GFP in HEK293 nuclear lysate expressing GFP. 20µg of lysate was incubated with primary antibody (1 µg/mg lysate) and matrix (Protein G) for 16 hours at 4°C in AFC low salt buffer. For western blotting ab290 (1/5000) was used to confirm successful immunoprecipation.

    Lane 1: HEK293 nuclear lysate expressing GFP input.
    Lane 2: IP of HEK293 nuclear lysate expressing GFP.
    Lane 3: Cells with no GFP.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    ab290 staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab290 at 1/200 dilution overnight at +4°C followed by incubation with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081), for 1 hour, at 1μg/ml.

    Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab290 was applied gave a stronger signal in the 488 channel, indicating that ab290 is binding to GFP and therefore eliciting signal amplification.

    ab290 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP. Nuclear DNA was labelled with 1.43μM DAPI (blue).

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290) This image is courtesy of an Abreview submitted by Vladimir Milenkovic

    ab290 immunoprecipitate in human HEK293 cells transfected with Annexin1-GFP. 25µg of cell lysate was incubated with the primary antibody and matrix (Protein G) in 1% TX-100, 10% glycerol, 1X PBS for 16 hours at 4°C. For Western blotting anti-rabbit HRP conjugated secondary antibody was used at a dilution at 1/5000.

    Lane 1: Lysate of HEK293 cells expressing Annexin1-GFP fusion protein.
    Lane 2: IP with anti-GFP.
    Lane 3: Not bound fraction.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290) This image is courtesy of an anonymous Abreview

    ab290 staining GFP in U2OS cells expressing TRF2-GFP fusion protein by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP40 and blocked with 3% BSA for 1 hour at 21°C. Samples were incubated with the primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at a dilution of 1/500 was used as the secondary antibody.

    Green - GFP.
    Blue - DAPI.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)
    Anti-GFP antibody (ab290) at 1/2500 dilution + Recombinant A. victoria GFP protein (ab84191) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Secondary antibody - goat anti-rabbit HRP preadsorbed (ab97080)

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290) This image is courtesy of an anonymous Abreview
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : LNCaP whole cell lysate - pEGFP empty vector
    Lane 2 : LNCaP whole cell lysate - pEGFP-PKD1 transfected

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 23°C.

    Incubated with the primary antibody for 16 hours at 4°C.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290) This image is courtesy of an Abreview submitted by S Houtman
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : COS7 whole cell lysate - transfected with GFP-Eml4
    Lane 2 : COS7 whole cell lysate - transfected with GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated pig anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 20°C.

    Incubated with the primary antibody for 18 hours at 4°C in TBS containing 2% milk and 1% Tween.

    Predicted MW of Eml4 ~ 120 kDa.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GFP antibody (ab290)

  •  
  • Product image

    Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    HRP Anti-GFP antibody [E385] (ab190584)

    Applications: WB

  •  
  • Product image

    Anti-GFP antibody [E385] (ab32146)

    Applications: WB

  •  
  • Product image

    Anti-GFP antibody [EPR14104-89] (ab183735)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-GFP antibody [E385] - BSA and Azide free (ab239807)

    Applications: WB

  •  
  • Product image

    Anti-GFP antibody [EPR14104] - BSA and Azide free (ab220802)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-GFP antibody [EPR14104] (ab183734)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.