Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Anti-GAPDH antibody - Loading Control (ab9485)

Price and availability

324 188 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-GAPDH antibody - Loading Control (ab9485)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GAPDH - Loading Control
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-HIP1R antibody (ab234991)
Product image
Anti-Huntingtin antibody - C-terminal (ab83915)
Product image
HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
Product image
Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)

Overview

  • Product name

    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description

    Rabbit polyclonal to GAPDH - Loading Control
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Human GAPDH.

Images

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab9485 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485) Image from Wu T et al., PLoS One, 14(4), Fig 3.; doi: 10.1371/journal.pone.0216042. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    HEK293 cells stably transfected with pINDUCER10-shNF90/NF110 (D2) or pINDUCER10-shNF45 (D5) were treated without or with doxycycline for 96 h, then serum starved for 12 h and treated with PMA (20 ng/mL) for 2 h. Protein lysates (20 µg/lane) were separated by SDS-PAGE and transferred to PVDF membranes. 

    Loading control: Rabbit polyclonal to GAPDH (ab9485) at 1/1000 dilution.

    Secondary antibodies (HRP) were used at 1/10,000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)

    IHC image of ab9485 staining GAPDH in human pancreas formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9485, 5μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HEK-293 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    Western blot image using 4-20% Optiblot gel with the Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623).
    20ug of Lysate per lane and detection using ab9485 diluted to 1ug/ml.
    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HEK-293 cell lysate
    Lane 5: HepG2 cell lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485) This image is courtesy of an anonymous Abreview
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution

    Lane 1 : Mouse hepatocytes - untreated
    Lane 2 : Mouse hepatocytes - treated with LPS (100 ng/mL) for 1 hour
    Lane 3 : Mouse hepatocytes - treated with LPS (100 ng/mL) for 12 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit secondary antibody (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 1 minute


    Primary incubation: 16 hours at 4°C

    Blocking: 5% milk for 1 hour at room temperature

     

    See Abreview

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485) This image is a courtesy of Anonymous Abreview
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution

    Lane 1 : Lysate prepared from human Huh-7 cells at 2 µg
    Lane 2 : Lysate prepared from human Huh-7 cells at 20 µg

    Secondary
    All lanes : HRP-conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 staining GAPDH in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min),  permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab195889 at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution + Mouse Embryonic lung whole tissue lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa


    Exposure time: 15 seconds

    See Abreview

  • ELISA - Anti-GAPDH antibody - Loading Control (ab9485)
    ELISA - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 detecting GAPDH in Human fibroblasts samples by indirect ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/2500) for 1 hour at 25°C. An alkaline phosphatase Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    The capture of ab8245 was 1/2000. Detection of ab9485 was 1/2500.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GAPDH antibody - Loading Control (ab9485)

  •  
  • Product image

    Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)

    Applications: WB

  •  
  • Product image

    Alexa Fluor® 555 Anti-GAPDH antibody [EPR16884] (ab206629)

    Applications: ICC/IF

  •  
  • Product image

    Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.