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Immunology Immune Interventions Transplantation

Anti-FKBP51 antibody [EPR6617] (ab126715)

Price and availability

324 876 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-FKBP51 antibody [EPR6617] (ab126715)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6617] to FKBP51
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-FKBP51 antibody [EPR6617]
    See all FKBP51 primary antibodies
  • Description

    Rabbit monoclonal [EPR6617] to FKBP51
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human FKBP51 aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: Q13451

  • Positive control

    • IHC-P: Human colon, Rat stomach and Human prostatic hyperplasia tissue. WB: Human testis tissue; Jurkat, HepG2, Caco-2 and HeLa whole cell lysate (ab150035). Wild-type HAP1 whole cell lysate. ICC/IF: Jurkat cells. Flow Cyt: HeLa cells. IP: Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6617
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune Interventions
    • Transplantation
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Images

  • Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126715 observed at 51 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126715 was shown to recognize FKBP51 in wild-type cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. Ab126715 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)
    All lanes : Anti-FKBP51 antibody [EPR6617] (ab126715) at 1/10000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg
    Lane 2 : Rat spleen lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunoprecipitation - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Immunoprecipitation - Anti-FKBP51 antibody [EPR6617] (ab126715)

    ab126715 (purified) at 1:20 dilution (2µg) immunoprecipitating FKBP51 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab126715 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126715 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [EPR6617] (ab126715)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling FKBP51 with purified ab126715 at 1:100 dilution (2.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    Alexa Fluor® 488 (ab198978) and Alexa Fluor® 647 (ab198979) conjugated versions are available for this clone.

  • Flow Cytometry - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Flow Cytometry - Anti-FKBP51 antibody [EPR6617] (ab126715)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FKBP51 with purified ab126715 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    Alexa Fluor® 488 (ab198978) and Alexa Fluor® 647 (ab198979) conjugated versions are available for this clone.

  • Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Western blot - Anti-FKBP51 antibody [EPR6617] (ab126715)
    All lanes : Anti-FKBP51 antibody [EPR6617] (ab126715) at 1/1000 dilution

    Lane 1 : Human testis cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] (ab126715)

    ab126715, at 1/50 dilution, stains FKBP51 in paraffin embedded human colon tissue by immunohistochemistry.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-FKBP51 antibody [EPR6617] (ab126715)
    OI-RD Scanning - Anti-FKBP51 antibody [EPR6617] (ab126715)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FKBP51 antibody [EPR6617] (ab126715)
    Anti-FKBP51 antibody [EPR6617] (ab126715)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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