Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Developmental Biology Lineage specification Endoderm

Anti-CDX2 antibody [EPR2764Y] (ab76541)

Price and availability

361 000 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-CDX2 antibody [EPR2764Y] (ab76541)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2764Y] to CDX2
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Anti-Ganglioside GD3 antibody [R24] (ab11779)
Product image
Recombinant Human Stra6 protein (ab163804)
Product image
Anti-Stra6 antibody (ab77616)
Product image
Recombinant Human CDX2 protein (ab114247)

Overview

  • Product name

    Anti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2764Y] to CDX2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CDX2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q99626

  • Positive control

    • Purchase matching WB positive control:Recombinant Human CDX2 protein
    • ICC/IF: Human colon and SW480 cells; Trophoblast stem (TS) cells. WB: Caco-2, HT-29, human, mouse and rat colon cancer and human small intestine lysates. IHC-P: Human colon, gastric adenocarcinoma, colonic adenocarcinoma, intestinal tissues; Rat and mouse colon tissues. Flow Cyt: HT-29 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Dissociation constant (KD)

    KD = 1.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2764Y
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Trophectoderm

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with Purified ab76541 at 1:500 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Flow Cytometry - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with Purified ab76541 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    All lanes : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/10000 dilution (Purified)

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human colon cancer lysates
    Lane 3 : Rat colon lysates
    Lane 4 : Mouse colon lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
    was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
    was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541) Ohinata et al PLoS One. 2014 Sep 9;9(9):e107308. doi: 10.1371/journal.pone.0107308. eCollection 2014. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Derivation of TS cells in CDM/FAXY.

    (Panel e)

    Immunofluorescence staining of TS (trophoblast stem) cells staining CDX2 using unpurified ab76541 on a fibronectin-coated plastic-bottom dish.

    Cells were grown for 2 days on a fibronectin-coated plastic-bottom dish, fixed with 4% paraformaldehyde/phosphate buffer for 1 hour at room temperature, and then washed three times with 0.1% Triton X100/PBS. Cells were blocked with blocking solution (0.5% normal goat serum in PBS +0.1% Triton X-100) and incubated overnight at 4°C with primary antibody diluted in blocking solution. Cells were then washed three times with PBST, incubated with Alexa Fluor®568–conjugated goat anti–rabbit IgG antibody {"type":"entrez-nucleotide","attrs":{"text":"A11036","term_id":"492396","term_text":"A11036"}} for 1 hour at room temperature, washed in PBST, counterstained with DAPI 1 µg/ml, and imaged. ab76541 was used at 1∶500 dilution.

    Scale bar, 100 µm.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541) This image is courtesy of an anonymous Abreview

    Unpurified ab76541 staining CDX2 in human colon cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution (Unpurified)
    Lanes 2-3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution (Unpurified)

    Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) lysate
    Lane 2 : Colon cancer lysate
    Lane 3 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541) This image is courtesy of an anonymous Abreview

    Unpurified ab76541 staining CDX2 in human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing positive staining in normal colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing positive staining in colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing negative staining in skeletal muscle tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing negative staining in normal kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)

    Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    OI-RD Scanning - Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CDX2 antibody [EPR2764Y] (ab76541)
    Anti-CDX2 antibody [EPR2764Y] (ab76541)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CDX2 antibody [EPR2764Y] (ab76541)

  •  
  • Product image

    Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Applications: Flow Cyt, ICC/IF, IHC-P

  •  
  • Product image

    Anti-CDX2 antibody (ab70458)

    Applications: IHC-P

  •  
  • Product image

    Anti-CDX2 antibody [CDX2/1690] (ab217884)

    Applications: IHC-P

  •  
  • Product image

    Anti-CDX2 antibody [PCRP-CDX2-1A3] - BSA and Azide free (ab269791)

    Applications: IHC-P, Protein Array

  •  
  • Product image

    Anti-CDX2 antibody [3G9B9] (ab181488)

    Applications: Flow Cyt, WB

  •  
  • Product image

    Anti-CDX2 antibody [EPR2764Y] - BSA and Azide free (ab220799)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-CDX2 antibody - N-terminal (ab220070)

    Applications: ICC/IF

  •  
  • Product image

    Anti-CDX2 antibody [SP54] (ab101532)

    Applications: Flow Cyt, ICC/IF, IHC-P

  •  
  • Product image

    Anti-CDX2 antibody [CDX2-88] (ab157524)

    Applications: IHC-P

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.